Journal: Biology

Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention

doi: 10.3390/biology15090719

Figure Lengend Snippet: Effects of GPE on osteoblast differentiation of human MSCs. BMSCs and AdMSCs were cultured in basal medium (CTR) or osteogenic differentiation medium (OM) with or without GPE (1–10 µg GAE/mL) for up to 21 days, and matrix mineralization was analyzed by Alizarin Red S staining. Representative light microscopic images of Alizarin Red S staining in BMSCs ( A ) and AdMSCs ( B ) and quantitative analysis ( C ). Each experiment was performed in triplicate. All data are presented as mean ± standard deviation (SD). # p < 0.01 versus undifferentiated control cells (CTR); * p < 0.05 versus osteogenic differentiated cells (OM).

Article Snippet: Human bone marrow-derived mesenchymal stem cells (BMSCs) from healthy donors (ATCC-PCS-500-012) were purchased from ATCC (Milan, Italy) as well as mesenchymal stem cell basal medium (ATCC PCS500030) and mesenchymal stem cell growth kit for BMSC (ATCC PCS500041).

Techniques: Cell Culture, Staining, Standard Deviation, Control

Journal: Biology

Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention

doi: 10.3390/biology15090719

Figure Lengend Snippet: Effects of GPE on the adipogenic differentiation of human MSCs. BMSCs and AdMSCs were cultured in basal medium (CTR) or adipogenic differentiation medium (AM) with or without GPE (1–10 µg GAE/mL) for up to 21 days, and lipid droplet formation was analyzed by Oil Red O (ORO) staining. Representative light microscopic images of ORO staining in BMSCs ( A ) and AdMSCs ( B ) and quantitative analysis ( C ). Each experiment was performed in triplicate. All data are presented as mean ± standard deviation (SD). # p < 0.01 versus undifferentiated control cells (CTR); * p < 0.05 and ** p < 0.01 versus adipogenic differentiated cells (AM).

Article Snippet: Human bone marrow-derived mesenchymal stem cells (BMSCs) from healthy donors (ATCC-PCS-500-012) were purchased from ATCC (Milan, Italy) as well as mesenchymal stem cell basal medium (ATCC PCS500030) and mesenchymal stem cell growth kit for BMSC (ATCC PCS500041).

Techniques: Cell Culture, Staining, Standard Deviation, Control

Journal: Scientific Reports

Article Title: Bone mesenchymal stem cells-derived exosomal miR-509-5p promotes osteogenic differentiation by targeting SFRP1 and activating the Wnt/β-catenin pathway

doi: 10.1038/s41598-025-33075-7

Figure Lengend Snippet: Identification of BMSCs and BMSCs-derived Exos. ( A ) Alkaline phosphatase and Alizarin red S were used to determine the multipotential differentiation capabilities of BMSCs; ( B ) Characteristic surface markers of BMSCs evaluated by flow cytometry; ( C ) The morphology of exosome (100 μm) was observed under a TEM; ( D ) Nanoparticle tracing assay of the BMSCs-derived Exos; ( E ) The protein levels of CD9, CD63, CD81, TSG101, and Hsp70 were measured by Western blot. Data are presented as mean ± SD ( n = 3 independent biological replicates).

Article Snippet: Human bone mesenchymal stem cells (BMSCs) obtained from Procell Biotechnology Co. LTD (Wuhan, China) were cultured in commercial BMSCs complete medium (Procell) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C and 5% CO 2 .

Techniques: Derivative Assay, Flow Cytometry, Western Blot

Journal: Scientific Reports

Article Title: Bone mesenchymal stem cells-derived exosomal miR-509-5p promotes osteogenic differentiation by targeting SFRP1 and activating the Wnt/β-catenin pathway

doi: 10.1038/s41598-025-33075-7

Figure Lengend Snippet: BMSCs-derived exosomes promoted osteogenic differentiation in hFOB1.19 cells via activation of the Wnt/β-catenin signaling pathway. ( A ) ALP staining was performed to assess osteogenic differentiation in each group; ( B ) Alizarin red S staining was performed to detect the mineralized area in each group; Relative mRNA and protein levels of RUNX2, OCN, OPN, Osterix, and ALP in each group were analyzed by ( C ) RT-qPCR and ( D ) Western blot; ( E ) Western blot was performed to detect the protein levels of Wnt1, β-catenin, GSK3β, and Cyclin D1 in each group. Data are presented as mean ± SD ( n = 3 independent biological replicates). *** p < 0.001 vs. the BMSCs-PBS group, ### p < 0.001 vs. the BMSCs-Exo group.

Article Snippet: Human bone mesenchymal stem cells (BMSCs) obtained from Procell Biotechnology Co. LTD (Wuhan, China) were cultured in commercial BMSCs complete medium (Procell) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C and 5% CO 2 .

Techniques: Derivative Assay, Activation Assay, Staining, Quantitative RT-PCR, Western Blot

Journal: Scientific Reports

Article Title: Bone mesenchymal stem cells-derived exosomal miR-509-5p promotes osteogenic differentiation by targeting SFRP1 and activating the Wnt/β-catenin pathway

doi: 10.1038/s41598-025-33075-7

Figure Lengend Snippet: Inhibition of BMSCs-derived exosomal miR-509-5p impaired osteogenic differentiation via suppression of the Wnt/β-catenin pathway in hFOB1.19 cells. ( A ) miRNA qPCR array-based detection of differentially expressed miRNAs between the BMSCs-PBS and the BMSCs-derived exosomes group; ( B ) The expression of miR-15b-5p, miR-24-3p, miR-148a-3p, miR-181b-5p, and miR-509-5p in BMSCs-PBS and the BMSCs-derived exosomes group were detected by RT-qPCR; ( C ) RT-qPCR was performed to assess the mRNA level of miR-15b-5p after miR-15b-5p inhibition; ( D ) ALP staining was performed to assess osteogenic differentiation in each group; ( E ) Alizarin red S staining was performed to detect the mineralized area in each group; ( F-G ) Relative mRNA and protein levels of RUNX2, OCN, OPN, Osterix, and ALP in each group were analyzed by RT-qPCR and Western blot; ( H ) Western blot was performed to detect the protein levels of Wnt1, β-catenin, GSK3β, and Cyclin D1 in each group. Data are presented as mean ± SD ( n = 3 independent biological replicates). * p < 0.05, *** p < 0.001 vs. the PBS group, ### p < 0.001 vs. the BMSCs-Exo + NC inhibitor group.

Article Snippet: Human bone mesenchymal stem cells (BMSCs) obtained from Procell Biotechnology Co. LTD (Wuhan, China) were cultured in commercial BMSCs complete medium (Procell) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C and 5% CO 2 .

Techniques: Inhibition, Derivative Assay, Expressing, Quantitative RT-PCR, Staining, Western Blot

Journal: Scientific Reports

Article Title: Bone mesenchymal stem cells-derived exosomal miR-509-5p promotes osteogenic differentiation by targeting SFRP1 and activating the Wnt/β-catenin pathway

doi: 10.1038/s41598-025-33075-7

Figure Lengend Snippet: Overexpression of BMSCs-derived exosomal miR-509-5p promoted osteogenic differentiation via activation of the Wnt/β-catenin pathway in hFOB1.19 cells. ( A ) RT-qPCR was performed to assess the mRNA level of miR-15b-5p after miR-15b-5p overexpression; ( B ) ALP staining was performed to assess osteogenic differentiation in each group; ( C ) Alizarin red S staining was performed to detect the mineralized area in each group; ( D-E ) Relative mRNA and protein levels of RUNX2, OCN, OPN, Osterix, and ALP in each group were analyzed by RT-qPCR and Western blot; ( F) Western blot was performed to detect the protein levels of Wnt1, β-catenin, GSK3β, and Cyclin D1 in each group. Data are presented as mean ± SD ( n = 3 independent biological replicates). ** p < 0.01, *** p < 0.001 vs. the miR-NC or the BMSCs-PBS group, ### p < 0.001 vs. the BMSCs-Exo + miR-NC group.

Article Snippet: Human bone mesenchymal stem cells (BMSCs) obtained from Procell Biotechnology Co. LTD (Wuhan, China) were cultured in commercial BMSCs complete medium (Procell) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C and 5% CO 2 .

Techniques: Over Expression, Derivative Assay, Activation Assay, Quantitative RT-PCR, Staining, Western Blot

Journal: Journal of Materials Science. Materials in Medicine

Article Title: Copper tannic acid coordination nanosheet as a potent in-situ antibiotic sustained-release carrier for chronic osteomyelitis

doi: 10.1007/s10856-025-06979-z

Figure Lengend Snippet: The effect of Van@CuTA on BMSCs and HUVECs. A CCK-8 assay demonstrated no toxicity of Van@CuTA toward BMSCs. B Representative images of alizarin red staining, revealing increased calcium deposition in BMSCs treated with Van@CuTA. C , D Representative images and statistical analysis of HUVECs migration assay demonstrating a significant increase in migrated cells following Van@CuTA treatment. E – G Tube formation assay images and corresponding quantifications of total tube length and node number per view indicated that Van@CuTA significantly promoted HUVECs’ tube formation. (* indicated p < 0.05)

Article Snippet: Primary bone marrow mesenchymal stem cells (BMSCs) and Human Umbilical Vein Endothelial Cells (HUVECs) were obtained from ATCC.

Techniques: CCK-8 Assay, Staining, Migration, Tube Formation Assay